Potential of Conditioned Medium of hATMSCs in Aging Cells Model

Skin aging is caused by the exposure cumulative of ultraviolet radiation, it leads reactive oxygen species (ROS) production in skin. The conditioned medium human Adipose Tissue-derived Mesenchymal Stem Cells (hATMSCs) can scavenge free radicals and increase survival rate skin cells under oxidative stress. This study examined protective effects Conditioned Medium (CM) hATMSCs H2O2-induced fibroblast cell line (BJ). model using BJ were added CM-hATMSCs concentrations (0, 10, 30%) incubated various time, furthermore induced H2O2 50, 100, 200 µM) for 1 h. anti-aging potential measured including viability, ROS collagen levels which treated CM-hATMSCs. median inhibitory concentration (IC50) on h incubation was 107.87 μM 91.25 10 min incubation. increased viability cells. 30% µM-induced 25% decreased ROS, level cells, decrease